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Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes

  • [设施]:实验1
  • [期刊/会议名称]:ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • [摘要]:The diversity and properties of actinobacteria, predominant residents in coral holobionts, have been rarely documented. In this study, we aimed to explore the species diversity, antimicrobial activities and biosynthetic potential of culturable actinomycetes within the tissues of the scleractinian corals Porites lutea, Galaxea fascicularis and Acropora millepora from the South China Sea. A total of 70 strains representing 13 families and 15 genera of actinobacteria were isolated. The antimicrobial activity and biosynthetic potential of fifteen representative filamentous actinomycetes were estimated. Crude fermentation extracts of 6 strains exhibited comparable or greater activities against Vibrio alginolyticus than ciprofloxacin. Seven of the 15 actinomycetes strains possess type I polyketide synthases (PKS-I) and/or nonribosomal peptide synthetases (NRPS) genes. Nine tested strains possess type II polyketide synthases (PKS-II). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these PKS and NRPS gene screening positive strains belong to genera Nocardiopsis, Pseudonocardia, Streptomyces, Micromonospora, Amycolatopsis and Prauserella. One PKS-I and four NRPS fragments showed < 70 % similarity to their closest relatives, which suggested the novelty of these genes. This study helps uncover the genetic capacity of stony coral-associated actinomycetes to produce bioactive molecules.
  • [发表日期]:2014
  • [第一作者]:Li, J
  • [第一作者单位]:Chinese Academy of Sciences
  • [通讯作者]:Zhang, S
  • [通讯作者单位]:Chinese Academy of Sciences
  • [论文类型]:期刊
  • [期刊分类]:MICROBIOLOGY(SCI3区)
  • [学科分类]:
  • [影响因子]:1.806
  • [关键词]:Scleractinian coral; Actinomycetes; Antimicrobial activity; PKS; NRPS
  • [卷号]:4
  • [期号]:106
  • [起止页码]:623-635
  • [简介]: