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Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities

  • [设施]:“科学”号海洋科学综合考察船
  • [期刊/会议名称]:Scientific Reports
  • [摘要]:A multifunctional enzyme is one that performs multiple physiological functions, thus benefiting the organism. Characterization of multifunctional enzymes is important for researchers to understand how organisms adapt to different environmental challenges. In the present study, we report the discovery of a novel multifunctional enzyme Amy63 produced by marine bacterium Vibrio alginolyticus 63. Remarkably, Amy63 possesses amylase, agarase and carrageenase activities. Amy63 is a substrate promiscuous α-amylase, with the substrate priority order of starch, carrageenan and agar. Amy63 maintains considerable amylase, carrageenase and agarase activities and stabilities at wide temperature and pH ranges, and optimum activities are detected at temperature of 60 °C and pH of 6.0, respectively. Moreover, the heteroexpression of Amy63 dramatically enhances the ability of E. colito degrade starch, carrageenan and agar. Motif searching shows three continuous glycosyl hydrolase 70 (GH70) family homologs existed in Amy63 encoding sequence. Combining serial deletions and phylogenetic analysis of Amy63, the GH70 homologs are proposed as the determinants of enzyme promiscuity. Notably, such enzymes exist in all kingdoms of life, thus providing an expanded perspective on studies of multifunctional enzymes. To our knowledge, this is the first report of an amylase having additional agarase and carrageenase activities.
  • [发表日期]:2016
  • [第一作者]:孙超岷
  • [第一作者单位]:中国科学院海洋研究所
  • [通讯作者]:孙超岷
  • [通讯作者单位]:中国科学院海洋研究所
  • [论文类型]:0
  • [期刊分类]:SCI1区
  • [学科分类]:交叉学科
  • [影响因子]:5.2
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