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Phospholipase Dα1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds

  • [设施]:种质库
  • [期刊/会议名称]:Plant, Cell and Environment上
  • [摘要]:High sensitivity of seeds to water loss is a widespread phenom- enon in the world’s plant species. The molecular basis of this traitispoorlyunderstoodbutthoughttobeassociatedwithcrit- ical changes in membrane function. We profiled membrane lipidsofseedsineightspecieswith varying levels of desiccation tolerance and found a close association between reducing seed viability and increasing phosphatidic acid (PA). We applied hydration–dehydration cycles to Arabidopsis seeds, which are normallydesiccationtolerant,tomimictheonset of desiccation sensitivitywithprogressiontowardsgerminationandexamined the role of phospholipase D (PLD) in desiccation stress- induced production of PA. We found that PLDα1 became more abundant and migrated from the cytosol to the mem- brane during desiccation, whereas PLDδ did not change, and that all desiccation-induced PA was derived from PLDα1 hy- drolysis. When PLDα1 was suppressed, the germination level after each hydration–dehydration cycle improved significantly. We further demonstrated that PLDα1-mediated PA formation modulates desiccation sensitivity as applying its inhibitor im- proved seed desiccation tolerance and its suppression in proto- plasts enhanced survival under dehydration. The insights provided by comparative lipidomics enable us to propose a new membrane-based model for seed desiccation stress and survival.
  • [发表日期]:2017
  • [第一作者]:Hongying Chen
  • [第一作者单位]:中国科学院昆明植物研究所
  • [通讯作者]:, Hugh W. Pritchard & Weiqi Li
  • [通讯作者单位]:英国皇家植物园丘园&中国科学院昆明植物研究所
  • [论文类型]:0
  • [期刊分类]:
  • [学科分类]:null
  • [影响因子]:6.443
  • [关键词]: drought; membrane lipid; phosphatidic acid (PA); phospholipase D (PLD); seed storage behaviour; water relation.
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