Equipped with 405nm, 488nm, 561nm, and 633nm lasers and PMT detectors, the inverted confocal microscopy imaging system not only meets the requirements of conventional multi-color/multi-layer/time-series fluorescence confocal imaging, but also supports various experiments such as fluorescence co-localization, fluorescence resonance energy transfer (FRET), fluorescence repair after photobleaching (FRAP), and spectral scanning.