The live-cell fluorescent super-resolution microscope endstation is to label the target structure with fluorescence probe,to illuminate the fluorescent sample with laser of suitable wavelength,and combine with four PI imaging technology,to realize single cell three-dimensional super-resolution imaging. It can be used to observe cell and tissue morphology,subcellular structure, etc. Further combined with X-ray imaging technology,it can realize the function of ultrastructure and fluorescence,in-situ composite imaging.