Radiation Terminal of Biology and Shallow-seated Tumor Therapy (TR4) is composed of irradiation equipment, sample changer, beam monitoring system, control system and radiation protection system. It is possible to use this facility to conduct mutation breeding induced by ion beams with different physical parameters (ion species, energy, linear energy transfer and dose rates, etc.) and the fundamental researches of carbon ion tumor therapy. The TR4 terminal is based semi-automatic platform which can perform irradiations with accurate dose and efficient sample changing. Not only does TR4 terminal perform the clinical studies of shallow-seated tumor therapy, but it can also satisfy the irradiation requirements of various biological samples (the seeds, tissue and shoots of plant; mammalian cells and small animals; the suspension and spores of microbes).